Metabolic Peptide Research Protocol

Each metabolic peptide operates through a distinct mechanism. AOD-9604 is a modified fragment of human growth hormone (hGH 176-191) that stimulates lipolysis and inhibits lipogenesis without affecting IGF-1 or insulin levels. MOTS-c is a mitochondrial-derived peptide that activates AMPK, enhances glucose uptake, and improves insulin sensitivity through the folate-AICAR pathway. 5-Amino-1MQ inhibits NNMT (nicotinamide N-methyltransferase), an enzyme overexpressed in adipose tissue that contributes to metabolic dysfunction.

Before beginning any metabolic peptide research, establish comprehensive baseline measurements. Record body weight, body composition estimates (skinfold measurements or bioimpedance), waist and hip circumference, and any available metabolic biomarkers. Photograph under standardized lighting conditions. These baseline data are essential for meaningful before/after comparison.

Literature-based research doses: AOD-9604 at 250-500 mcg/day (subcutaneous, typically pre-fasted morning), MOTS-c at 5-10 mg administered 2-3 times per week (subcutaneous), 5-Amino-1MQ at 50-100 mg/day (oral, with or without food). Not all three need to be used simultaneously — researchers may study individual compounds or select combinations based on their specific research question.

Create a structured weekly schedule. A common research framework: AOD-9604 daily (morning, fasted), MOTS-c on Monday/Wednesday/Friday (any time), 5-Amino-1MQ daily (oral, morning). If studying compounds individually, maintain the same dosing schedule for that compound alone. Document the schedule clearly and adhere to consistent timing.

For AOD-9604 and MOTS-c, follow the Subcutaneous Injection Technique Protocol. Abdominal subcutaneous injection is the standard site for metabolic peptide research. Rotate injection sites to prevent lipodystrophy. Use separate syringes for each peptide.

Collect measurements weekly using the same protocol as baseline. At minimum, record body weight, waist circumference, and subjective observations. Monthly measurements should include full body composition assessment and photographs. Note any changes in energy levels, appetite, exercise capacity, or other subjective parameters.

After the planned research period (typically 8-12 weeks), compile all measurement data into a summary report. Compare against baseline measurements. Note which compounds were used, exact doses, adherence rate, and any confounding variables (dietary changes, exercise modifications). This documentation is essential for reproducibility.