Peptide Dosing Calculation Protocol

Before calculating a dose, you need three values: (1) the total peptide mass in the vial (in mg or mcg), (2) the total volume of bacteriostatic water used for reconstitution (in mL), and (3) your target dose (in mcg or mg). These should be recorded from your reconstitution protocol.

Divide the total peptide mass by the reconstitution volume to get the concentration. For example: 5 mg peptide in 2 mL water = 2.5 mg/mL = 2,500 mcg/mL. This is the concentration of your reconstituted solution.

Divide your target dose by the solution concentration to get the required volume. For example: if you need 250 mcg and your concentration is 2,500 mcg/mL, you need 0.1 mL (250 / 2,500 = 0.1 mL).

A standard U-100 insulin syringe holds 1 mL total, divided into 100 units. Each unit marking equals 0.01 mL. So 0.1 mL = 10 units. Draw to the appropriate unit marking on your syringe.

Swab the vial stopper with alcohol. Insert the syringe needle and draw slightly past your target volume. Tap the syringe to move air bubbles to the top, then push the plunger to your exact target marking while expelling air. Verify the volume at eye level.

Log the date, time, peptide name, dose (mcg/mg), volume drawn (mL/units), and any observations in your research notebook. Tracking cumulative doses and remaining vial volume ensures accuracy throughout your research.