Biochemistry

    Molecular Weight

    Definition

    Molecular weight (MW), also called molecular mass, is the sum of the atomic weights of all atoms in a molecule, expressed in Daltons (Da) or grams per mole (g/mol). For peptides, molecular weight is determined by the amino acid sequence and any post-translational modifications. It is a key identifier used in quality control — mass spectrometry confirms that a synthesized peptide matches its expected molecular weight. Peptides are generally defined as chains under 50 amino acids (roughly under 5,500 Da).

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