Formulation
Reconstitution
Definition
Reconstitution is the process of dissolving a lyophilized (freeze-dried) peptide powder back into solution using an appropriate solvent, typically bacteriostatic water or sterile water. Proper reconstitution technique is critical for research accuracy: the solvent should be added slowly along the vial wall, allowing the powder to dissolve without agitation, as vigorous shaking can damage peptide structures. The resulting solution concentration depends on the volume of solvent added.